First Report of Cherry Virus Turkey in Sweet Cherry in Greece

HomePlant DiseaseVol. 105, No. 1First Report of Cherry Virus Turkey in Sweet Greece PreviousNext DISEASE NOTES OPENOpen Access licenseFirst GreeceA. Katsiani, S. Li, J. Zhou, E. Demertzi, N. I. Katis, and V. MaliogkaA. Katsianihttp://orcid.org/0000-0003-4229-3449Laboratory Plant Pathology, School Agriculture, Faculty Forestry Natural Environment, Aristotle University Thessaloniki, 54124 GreeceSearch for more papers by this author, Lihttp://orcid.org/0000-0003-4614-1768State Key Laboratory Biology Diseases Insect Pests, Institute Protection, Chinese Academy Agricultural Sciences, Beijing, 100094, P.R. ChinaSearch ZhouState DemertziLaboratory KatisLaboratory Maliogka†Corresponding author: Maliogka; E-mail Address: [email protected]://orcid.org/0000-0001-5714-2710Laboratory author AffiliationsAuthors Affiliations A. Katsiani1 Li2 Zhou2 Demertzi1 Katis1 Maliogka1 † 1Laboratory 2State China Published Online:27 Oct 2020https://doi.org/10.1094/PDIS-05-20-1078-PDNAboutSectionsView articlePDFPDF Plus ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleThe number viruses identified sweet cherry has been constantly increasing over the last few years, following broad application high-throughput sequencing (HTS). Some these were reported cause leaf symptoms yield reduction. In 2009 2013, surveys performed on orchards located northern presence Betaflexiviridae using generic specific molecular assays (Foissac et al. 2005). Sanger a reverse transcription polymerase chain reaction (RT-PCR) product originated from sample collected symptomless (cv. Ferrovia) revealed virus sequence sharing 75% nucleotide (nt) similarity with highly conserved portion betaflexivirus gene. Total RNA, extracted TRIzol reagent (Invitrogen, Carlsbad, CA) leaves sample, was subjected HTS analysis an Illumina Hi-seq 4000 platform (Novogene, Tiangen, China). The run, after quality control, yielded 26,002,319 150-nt-long paired-end reads. De novo assembly reads Geneious Prime 2019.2.3 Velvet subsequent BLAST (BLASTn/x) 82 contigs revealed, apart several known (cherry A, Prunus F, little 1), newly described Robigovirus species. More specifically, results that eight de ranging 277 2,287 nt long similar recently (CVTR) (Çağlayan 2019). nearly complete genome consisting 8,362 (99.5%) Greek CVTR isolate (CVTR-C18GR, accession no. MT043307) reconstructed iterative mapping contigs, 4,690 mapped total RT-PCR amplicons fill gaps. Similarity CVTR-C18GR full sequences GenBank CVTR-BUR12 (MK600387) CVTRAZQ (MH177869) showed 90.47 90.60% identities, respectively. Phylogenetic analyses confirmed clustering C18GR other isolates. To investigate Greece, primers F2Rob (5′-GTCAGAGAGAGGTATCTATGTC-3′) RT1Rob (5′-CAGGCTGTTCATAACCT-3′), which amplify 577 replicase, designed. Sixty-six samples trees showing no virus-like various screened CVTR. Eight them found be infected new virus. C11GR amplicon fields (96% C18GR, MT043308). Sequence between isolates also high (97% nt) partial TGB2, TGB3, CP genomic regions amplified primer pair 7006 F (5′-GAAAAGTGATTATTCAGCRCCAGT-3′) 7708 R (5′-CTTTCACCCACTCATCACCTATCTCC-3′) (accession MT461407). This is first report thus extending information its geographical distribution. Robigoviruses variable diseases sour cherries, reason they are included European Commission Directive concerning official inspections (2019/2072/EU). Additional screenings necessary evaluate impact Greece.The author(s) declare conflict interest.References:Çağlayan, K., 2019. Pathogens 8:57. https://doi.org/10.3390/pathogens8020057 Crossref, Google ScholarFoissac, X., 2005. Phytopathology 95:617. https://doi.org/10.1094/PHYTO-95-0617 Link, ISI, ScholarThe interest.Funding: study partially funded VirFree. project received funding Union’s Horizon 2020 research innovation program under Marie Skłodowska-Curie grant agreement 734736. publication reflects only authors’ view. Agency not responsible any use may made it contains. Moreover, co‐financed Union national funds through Operational Program Competitiveness, Entrepreneurship Innovation, call RESEARCH – CREATE INNOVATE (project code: T1EDK-05438).DetailsFiguresLiterature CitedRelated Vol. 1 January 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionTypical star anise spot disease caused Alternaria tenuissima (J.-L. Lai al.). Photo credit: J.-L. Lai. Water drops rolling strawberry blade sporulating lesion powdery mildew (B. Asalf B. Asalf. Metrics Downloaded 723 times Article History Issue Date: 8 Jan 2021Published: 27 2020First Look: 15 Jul 2020Accepted: 10 Page: 235 Information© 2021 American Phytopathological SocietyFundingEuropean programGrant/Award Number: 734736Operational InnovationGrant/Award T1EDK-05438)KeywordsRobigovirus incidencenew virusHTScherry TurkeyThe interest.PDF downloadCited ByIdentification Analysis Novel Ilarvirus Infecting Cherry10 March | Plants, 10, 3